Process of making means for reducing blood-pressure



GEUIRG ZUELZER, OF CHARLOTTENBURG, NEAR BERLIN, GERM it ASSTGNOR, Ell

MESNE ASSIGNMENTS, TO THE CHEMICAL FOUNDATION, INC., A CORPORATION 0h nnnnwann.

PROCESS OF MAKING MEANS FOR REDUCING BLUOD-PRESSURE.

No Drawing. Y Application filed June 15,

To all whom it may concern 1 Be it known that l, Gnonc ZUnLznR, a citizen of the German Empire, and residing at Charlottenburg, near Berlin, Germany, have invented a certain new and useful Improved Process of Making Means for Reducing Blood-Pressure, of which the following is a specification.

My invention relates to preparations for reducing the blood pressure, and to processes of making the same.

lt is well known that albumoses are capabio of considerably reducing the blood pressure. As the diminution in the pressure takes place very rapidly however, the employment of albumoses for this purpose is attended with considerable danger to the health of the patient.

A primary object of my invention is to provide an improved preparation whose action on the blood pressure is less violent but occurs all the more continuously.

The following example will show how such a preparation according to my invention may be made 2 mg. bacterium coli, which have passed through a predetermined number of passages in the rabbit, are placed with standard or normal amboceptores for twelve hours inv the refrigerator, then liberated from the serum by severe centrifuging and repeatedly washed, the bacteria being killed prior to centrifuging by the bactericidal action of the amboceptor serum. The Washed sediment thus obtained is then put into 10 c. ems. 10% gelatin and is cooled after being mixed. The preparation is then ready for use. The preparation is administered preferably by injecting it intramuscularly into the body of the patient.

The amboceptors above mentioned are those immunizing bodies found in blood serum which are formed by inoculation of an animal, such as the rabbit, monkey, guinea pig, with bacilli. They are thermostable bodies possessin groups, namely, a-c'ytophile and complementOphile' one of which groups unites with a Specification of Letters Patent.

two haptophor Patented on. it thtl.

191a. serial No. erases.

bacillus destroying thereby its vitality and releasing the endotoxin or albumose content thereof.

Instead of using bacterium coli as descrlbed in the foregoin example, other saprophytic bacteria whlch readily yield endotoxin and which have been previously admixed with an excess of normal amboceptors greater than may be produced by the activity of the bacterial content, may be employed.

l claim:

1. A preparation for reducing blood pressure, composed of gelatin containing nonpathogenic bacteria which ftrm endotoxins charged with normal amboceptors, the amboceptor content being in excess of that which may be roduced through the activity of the bacteria content.

2. A preparation for reducing blood pressure, composed of gelatin containing bacterium coli charged with normal amboceptors, the amboceptor content being in excess of/ that which may be produced through the activity of the bacterial content.

3.. A process of making means for reducing blood pressure consisting in charging non-pathogenic bacteria which form endotoxins with normal amboceptors, and mixing the same with gelatin.

at. A process of making means for reducing blood pressure consisting in charging bacterium coli with normal amboceptors, and mixing the same with gelatin.

5. The process of making means for reducing blood pressure which comprises mixing non-pathogenic bacteria with normal amboceptors, refrigerating said mixture for about 12 hours, separating the bacteria and amboceptors from the liquid medium, washing the separated mass and incorporating said separated mass with gelatin.

6. The process of making means for re,- ducing blood pressure which comprises mixingrbacterium coli with normal amboceptors, re igerating said mixture for about 12 hours, separating the bacteria and amboceptors from the liquid medium, washing the arated mass with 10 0.0. of 10% finally cooling.

In testimony whereof, I affix my signature in the presence of two witnesses.

DR. GEORG ZUELZER.

gelatin, and 10 Witnesses WOLDEMAR HAUPT,

HENRY HABP'ER. 

